Can Cinnamaldehyde Increased the Innate Immune System on Medaka Larvae?

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Published Aug 8, 2020
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Vol. 2 No. 1 (2019): Volume 2 Issue 1

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Asthervina Widyastami Puspitasari
Faculty of Fisheries and Marine Science, University of Brawijaya, Indonesia
Diana Arfiati
Shao-Yang Hu

Abstract

Fish larvae are the early stage of the life cycle and the key to mass production of fish. Its nature which is susceptible to invade by pathogens, especially viruses, it is an important concern in increasing the innate immune system in virus invading. IFNa and Mx are inducer genes that have the most role in enhancing the innate immune system. The study was conducted in September 2017 with the experimental method. The sample used was 1-day-old medaka larvae in amount 30 fish in each tank with triplicate in each concentration. The cytotoxicity results showed that at 72 hours cinnamaldehyde immersion was 100% of medaka larvae survival rate at a concentration of 2.5 µg mL-1 and 0 µg mL-1 (control). One-Way ANOVA results, the genes expression of IFNa and Mx on 1-day-old medaka larvae showed decreasing at 2.5 µg mL-1 concentration of cinnamaldehyde immersion for 3 days with several observation periods (12h; 24h; 48h; and 72h) compared to control. The lowest expression of IFNa genes was 12h (2.54 x 10-5 ± 6.12 x 10-6) at a 2.5 µg mL-1concentration of cinnamaldehyde, and the highest expression of IFNa gene was 48h (3.95 x 10-4 ± 7.78 x 10-5)at 0 µg mL-1 (control)concentration of cinnamaldehyde. While the lowest Mx gene expression was 72h  (8.96 x 10-3 ± 4.51 x 10-4) at a 2.5 µg mL-1concentration of cinnamaldehyde. And the highest Mx gene expression was 72h (0.88 ± 0.26) at 0 µg mL-1 (control)concentration of cinnamaldehyde. The immersion of cinnamaldehyde extract at a concentration of  2.5 µg mL-1 is not able to increase the innate immune system in medaka larvae. More assay is needed to determine the mechanism for the phenomenon of decreasing IFNa and Mx gene expression on fish larvae.

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite
Puspitasari, A. W., Arfiati, D. . and Hu, S.-Y. (2020) “Can Cinnamaldehyde Increased the Innate Immune System on Medaka Larvae?”, Bioinformatics and Biomedical Research Journal, 2(1), pp. 21–26. Available at: http://bbrjournal.com/index.php/bbrj/article/view/78 (Accessed: 11December2023).

References

  1. Leung TL, Bates (2013) More rapid and severe disease outbreaks for aquaculture at the tropics: implications for food security. Journal of applied ecology, 50 (1), 215-222.
  2. Lafferty KD, Harvell CD, Conrad JM, Friedman CS, Kent ML, Kuris AM, et al. (2015) Infectious diseases affect marine fisheries and aquaculture economics. Annual review of marine science. 7:471- 96.
  3. Crane M, Hyatt A (2011) Viruses of fish: an overview of significant pathogens. Viruses. 3(11):2025-46.
  4. Stetson DB, Medzhitov R (2006) Type I interferons in host defense. Immunity. 25 (3):373-81.
  5. Sivasankar P (2017) A review on DNA viral diseases of fish. International Journal of Fisheries and Aquatic Studies. 5 (4): 443450.
  6. Uribe C, Folch H, Enríquez R, Moran G (2011) Innate and adaptive immunity in teleost fish: a review. Veterinarni Medicina. 56(10):486-503.
  7. El-Baky NA, Redwan EM (2015) Therapeutic alpha-interferons protein: structure, production, and biosimilar. Preparative Biochemistry and Biotechnology. 45(2):109-27.
  8. Staeheli P, Yu Y, Grob R, Haller O (1989) A double-stranded RNA-inducible fish gene homologous to the murine influenza virus resistance gene Mx. Molecular and Cellular Biology. 9(7):3117-21.
  9. Trobridge GD, Chiou PP, Leong J (1997) Cloning of the rainbow trout (Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein expression in salmon cells. Journal of virology. 71(7):5304-11.
  10. Bricknell I, Dalmo RA (2005) The use of immunostimulants in fish larval aquaculture. Fish & shellfish immunology. 19(5):45772.
  11. Chen B-J, Fu C-S, Li G-H, Wang X-N, Lou H-X, Ren D-M, et al. (2017) Cinnamaldehyde analogues as potential therapeutic agents. Mini reviews in medicinal chemistry. 17(1):33-43.
  12. Rao PV, Gan SH (2014) Cinnamon: a multifaceted medicinal plant. EvidenceBased Complementary and Alternative Medicine.
  13. Zhang Y, Li S, Kong X. (2013) Relationship between antimold activity and molecular structure of cinnamaldehyde analogues. Bioorganic & medicinal chemistry letters. 23(5):1358- 64.
  14. Yossa N, Patel J, Millner P, Lo YM. (2012) Essential oils reduce Escherichia coli O157: H7 and Salmonella on spinach leaves. Journal of food protection. 75(3):488-96.
  15. Visvalingam J, Hernandez-Doria JD, Holley RA (2013) Examination of the genome-wide transcriptional response of Escherichia coli O157: H7 to cinnamaldehyde exposure. Appl Environ Microbiol. 79(3):942-50.
  16. Kwon J, Yu C, Park H. (2003) Bacteriocidal effects and inhibition of cell separation of cinnamic aldehyde on Bacillus cereus. Letters in applied microbiology. 37(1):61-5.
  17. Friedman M. (2017) Chemistry, antimicrobial mechanisms, and antibiotic activities of Cinnamaldehyde against pathogenic bacteria in animal feeds and human foods. Journal of agricultural and food chemistry. 65(48):10406-23.
  18. Gill A, Holley R. (2006) Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. International journal of food microbiology. 108(1):1-9.
  19. Furutani-Seiki M, Sasado T, Morinaga C, Suwa H, Niwa K, Yoda H, et al. (2004). A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes. Mechanisms of development. 2004;121(7-8):647-58.
  20. Porazinski SR, Wang H, Furutani-Seiki M. (2010). Microinjection of medaka embryos for use as a model genetic organism. JoVE (Journal of Visualized Experiments). (46):e1937.
  21. Yamamoto TS (1964) Effects of Acidulated Ringer's Solution on the Egg of Dog Salmon, Oncorhynchus keta (With 2 Plates and 4 Tables). Journal of The Faculty of Science Hokkaido University Series VI Zoology. 15(3):426-38.
  22. Puspitasari AW, Arfiati D, Hu S-Y. (2018) Effect of Cinnamaldehyde Addition on Zebrafish (Danio rerio) Eggs Against Streptococcus agalactiae Infection. Research Journal of Life Science. 4(3):190-8.
  23. Maekawa S, Chiang Y-A, Hikima J-i, Sakai M, Lo C-F, Wang H-C, et al. (2016) Expression and biological activity of two types of interferon genes in medaka (Oryzias latipes). Fish & shellfish immunology. 48:20-29.
  24. National Research Council. (1993) Pesticides in the Diets of Infants and Children. Board on Agriculture and Board on Environmental Studies and Toxicology. National Academy Press. Washigton D. C.
  25. Rahemi D, Babaee N, Kazemi S, Sefidgar SAA, Moghadamnia AA (2015) An in vitro study of the effect of Cinnamaldehyde on the growth of Candida albicans compared to nystatin and fluconazole. 2(3):76-80
  26. Panawala, L (2017) Difference Between Bacteria and Virus. Research Gate.https://www.researchgate.net/publica tion/314214838
  27. Zhang Y-B, Gui J-F (2012) Molecular regulation of interferon antiviral response in fish. Developmental & Comparative Immunology. 38(2):193-202.
  28. Gan Z, Chen SN, Huang B, Zou J, Nie P. (2020). Fish type I and type II interferons: composition, receptor usage, production and function. Reviews in Aquaculture. 12(2):773-804.
  29. Schneider WM, Chevillotte MD, Rice CM (2014) Interferonstimulated genes: a complex web of host defenses. Annual review of immunology. 32:513-45.
  30. Verhelst J, Hulpiau P, Saelens X (2013) Mx proteins: antiviral gatekeepers that restrain the uninvited. Microbiol Mol Biol Rev. 77(4):551-66.
  31. Tort L (2011) Stress and immune modulation in fish. Developmental & Comparative Immunology. 35(12):1366- 75.
  32. Karnen GB, Iris R, eds. (2014) Imunologi Dasar. 4:106-10.
  33. Lee S-H, Vidal SM (2002) Functional diversity of Mx proteins: variations on a theme of host resistance to infection. Genome research. 12(4):527-30.
  34. Haller O, Staeheli P, Kochs G (2007) Interferon-induced Mx proteins in antiviral host defense. Biochimie. 89 (6- 7):812-8.
  35. Stranden AM, Staeheli P, Pavlovic J (1993) Function of the mouse Mx1 protein is inhibited by expression of the PB2 protein of influenza virus. Virology. 197(2):642-51.
  36. Kochs G, Haller O (1999) Interferoninduced human MxA GTPase blocks nuclear import of Thogoto virus nucleocapsids. Proceedings of the National Academy of Sciences. 96(5):20826.
  37. Sadler AJ, Williams BR (2008) Interferon-inducible antiviral effectors. Nature reviews immunology. 8(7):559. 38. Collet B (2014) Innate immune responses of salmonid fish to viral infections. Developmental & Comparative Immunology. 2014;43(2):160-73.
  38. Robertsen B (2008) Expression of interferon and interferon-induced genes in salmonids in response to virus infection, interferon-inducing compounds and vaccination. Fish & shellfish immunology. 25(4):351-7.
  39. Ingerslev HC, Rønneseth A, Pettersen E, Wergeland H (2009) Differential expression of immune genes in Atlantic salmon (Salmo salar L.) challenged intraperitoneally or by cohabitation with IPNV. Scandinavian Journal of Immunology.69(2):90-8.