IN VITRO TEST OF COMBINATION OF LEAF EXTRACTS AND BARK OF Xylocarpus granatum AS ANTISEPTIC CANDIDATES

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Published Feb 14, 2023
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Vol. 5 No. 1 (2022): Volume 5 Issue 1 (INPRESS)

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Lia Yulia Budiarti
Faculty of Medicine, Lambung Mangkurat University
Farida Heriyani
Department of Public Health, Faculty of Medicine, Lambung Mangkurat University Banjarmasin, Indonesia
Shinta Nuriyah
Faculty of Medicine, Lambung Mangkurat University

Abstract

Antiseptics can be made from plant extracts that are antibacterial. Parts of leaves and bark Xylocarpus granatum contains antibacterial compounds such assaponins, flavonoids, tannins, phenols, and alkaloid, so that it can be developed as a natural antiseptic candidate. This experimental study aims to analyze the activity of a combination of leaf and bark extracts X. granatum against several test bacteria in vitro. The plan uses a post-test only with control group design with a combination treatment of leaf ethanol extract (L) and bark (B) X. granatum 25%, 50%, 75%, and 100% (1:1 ratio) and 70% alcohol control. The dilution method was used to observe the activity of the extract with antiseptic control on the number of test bacterial colonies. Comparison of the effect as an antiseptic was analyzed using Duncan's ANOVA and post-hoc tests with a confidence level of 95%. L+B combination extract X. granatum produce significantly different inhibitory effects on the colonization of test bacteria; number of colonies S.aureus and S.epidermidis relatively less than E.coli, P.aeruginosa, S.typhi. L+B Extract X. granatum 100% produces an effect equivalent to 70% alcohol on all test bacteria, thus acting as an antiseptic candidate. in conclusion,a combination of leaf and stem bark extracts X. granatum has activity as an antiseptic against some test bacteria.


 

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How to Cite
Budiarti, L. Y. ., Heriyani, F. . and Nuriyah, S. . (2023) “IN VITRO TEST OF COMBINATION OF LEAF EXTRACTS AND BARK OF Xylocarpus granatum AS ANTISEPTIC CANDIDATES”, Bioinformatics and Biomedical Research Journal, 5(1), pp. 6–12. Available at: http://bbrjournal.com/index.php/bbrj/article/view/131 (Accessed: 11December2023).

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