Leaf and peel of the kaffir lime plant (Citrus hystrix D.C.) can be used as herbal preparations to treat infections by opportunistic bacteria. The purpose of this study was to analyze the antibacterial activity of the combination of leaf and peel extract against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa bacteria isolates. This study used a posttest-only control group design, including phytochemical tests and in vitro antibacterial activity tests. The combination of leaf and peel extract of kaffir lime tested was 12.5%, 25%, 50%, and 75% (w/v). The results of phytochemical tests on the leaf extract and rind of limes were found to have antibacterial compounds, namely flavonoids, tannins, phenols, and alkaloids. The results of the antibacterial activity test used the paper disc diffusion method, showed that there were differences in the size of the inhibition zone for the four test bacteria; The combination treatment of 75% (1:1) leaf-peel extract gave the greatest inhibitory zone effect on the four test bacteria and was equivalent to the positive control. The conclusion of the study, the combination of leaf and peel extract had different antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa.
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